ABSTRACT
Ischemic stroke is one of the leading causes of death and disability worldwide. A large number of preclinical studies have demonstrated that exogenous cell-based therapies such as mesenchymal stem cell transplantation can promote brain function recovery in the subacute phase of stroke. Emerging data indicate that mesenchymal stem cell-derived exosomes play a key role in mediating tissue repair by participating in intercellular signal transduction and transferring biological information especially microRNA to recipient cells, which affects endo-genous recovery in ischemic brain tissue after injury. In this review we briefly describe the characteristics and biological functions of exosomes and exosomal microRNA, and discuss the therapeutic effects of mesenchymal stem cell-derived exosomes on ischemic stroke from different perspectives. Finally, we outline the potential clinical value of exosomes and challenges of translating these therapies into clinical trials.
ABSTRACT
In this study, bioinformatics methods such as molecular docking and network pharmacology were adopted to establish Xiaoxuming Decoction (XXMD) "compound-vasodilatory and vasoconstrictory related G protein-coupled receptors (GPCR) targets" network, then the vascular function regulatory effective components and the potential targets of XXMD were analyzed. Based on the XXMD herb sources, the chemical structures of the compounds were retrieved from the national scientific data sharing platform for population and health pharmaceutical information center, TCMSP database and the latest research literatures. The chemical molecular library was established after class prediction and screening for medicinal and metabolic properties. Then, five kinds of vasodilatory and vasoconstrictory related GPCR crystal structure including 5-HT receptors (5-HT1AR, 5-HT1BR), AT1R, β2-AR, hUTR and ETB were retrieved from RCSB Protein Data Bank database or constructed by homology modeling of Discovery Studio 4.1 built-in modeling tools. After virtual screening by Libdock molecular docking, the highest rated 50 compounds of each target were collected and analyzed. The collected data were further used to construct and analyze the network by Cytoscape 3.4.0. The results showed that most of the chemical composition effects were associated with different vasodilatory and vasoconstrictory related GPCR targets, while a few effective components could be applied to multiple GPCR targets at the same time, therefore forming synergies and vasorelaxant effects of XXMD.
Subject(s)
Databases, Protein , Drugs, Chinese Herbal , Models, Chemical , Molecular Docking Simulation , Receptors, G-Protein-Coupled , Metabolism , VasodilationABSTRACT
OBJECTIVE Using bioinformatics methods, to establish Xiao-Xu-Ming decoction (XX-MD)"compound-vasoconstriction G Protein-Coupled Receptors(GPCR)targets"network,and analyze the vasoconstriction regulatory effective components and the potential targets of XXMD. METHODS Ac-cording to the XXMD herb sources,we retrieved the chemical structures from the national scientific da-ta sharing platform for population and health pharmaceutical information center,TCMSP database and the latest research literature.The chemical molecular library was established after class prediction and screening for medicinal and metabolic properties.Five kinds of vasoconstriction GPCR crystal structure including 5-HT receptors(5-HT1AR,5-HT1BR),AT1R,β2-AR,hUTR and ETB were retrieved from Bank Pro-tein Data Bank database or homology modeling using Discovery Studio 4.1 built-in modeling tools.After virtual screening by Libdock molecular docking,the highest rated 50 compounds of each target were col-lected and analyzed. The collected data were further used to construct and analyze the network. RE-SULTS 859 single compound structures information in XXMD were generalized following the screen-ing of obtained 2043 compounds.The complicated compound-vasoconstriction GPCR targets network of XXMD was then constructed and analyzed by molecular docking with the above five kinds of GPCR target receptors. Most of the chemical composition effects were associated with different vasoconstric-tion GPCR targets,while a few effective components can be applied to multiple GPCR targets at the same time,therefore forming synergies.CONCLUSION Vasorelaxant effects of XXMD may not only result from the collaborative interaction between a variety of active ingredients in Chinese medicine and multi-ple targets,but also from the interaction between some effective component and multiple targets.
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Objective To investigate the clinical effect of combination of traditional Chinese and western medicine on the treatment of senile multiple organ failure. Methods The control group of elderly patients with multiple organ failure was given routine western medicine treatment, and the study group was given the combination of Chinese and Western medicine treatment. Changes of BUN, PaO2, PaCO2, Cr, LVEF, CO and so on were recorded before and after treatment in two groups. Results There was no significant difference in comparison of related indexes of heart and kidney function value between two groups before treatment; after treatment in two groups, there were significantly decreased in BUN, PaCO2 and Cr index values compared with before, while PaO2, CO and LVEF significantly increased than before, index of the study group were improved better than those of the control group (P<0.05). Conclusion the application of conventional western medicine treatment of multiple organ failure in the elderly based on the use of Chinese medicine can significantly improve the clinical efficacy, to ensure the quality of life, life has important value in safety.
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Objective To investigate the clinical effect of combination of traditional Chinese and western medicine on the treatment of senile multiple organ failure. Methods The control group of elderly patients with multiple organ failure was given routine western medicine treatment, and the study group was given the combination of Chinese and Western medicine treatment. Changes of BUN, PaO2, PaCO2, Cr, LVEF, CO and so on were recorded before and after treatment in two groups. Results There was no significant difference in comparison of related indexes of heart and kidney function value between two groups before treatment; after treatment in two groups, there were significantly decreased in BUN, PaCO2 and Cr index values compared with before, while PaO2, CO and LVEF significantly increased than before, index of the study group were improved better than those of the control group (P<0.05). Conclusion the application of conventional western medicine treatment of multiple organ failure in the elderly based on the use of Chinese medicine can significantly improve the clinical efficacy, to ensure the quality of life, life has important value in safety.